Metadata-Version: 2.1
Name: DeepCell-CPU
Version: 0.9.1
Summary: Deep learning for single cell image segmentation
Home-page: https://github.com/vanvalenlab/deepcell-tf
Author: The Van Valen Lab
Author-email: vanvalen@caltech.edu
License: LICENSE
Download-URL: https://github.com/vanvalenlab/deepcell-tf/tarball/0.9.1
Description: # ![DeepCell Banner](https://raw.githubusercontent.com/vanvalenlab/deepcell-tf/master/docs/images/DeepCell_tf_Banner.png)
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        `deepcell-tf` is a deep learning library for single-cell analysis of biological images. It is written in Python and built using [TensorFlow 2](https://github.com/tensorflow/tensorflow).
        
        This library allows users to apply pre-existing models to imaging data as well as to develop new deep learning models for single-cell analysis. This library specializes in models for cell segmentation (whole-cell and nuclear) in 2D and 3D images as well as cell tracking in 2D time-lapse datasets. These models are applicable to data ranging from multiplexed images of tissues to dynamic live-cell imaging movies.
        
        `deepcell-tf` is one of several resources created by the [Van Valen lab](http://vanvalen.caltech.edu/) to facilitate the development and application of new deep learning methods to biology. Other projects within our DeepCell ecosystem include the [DeepCell Toolbox](https://github.com/vanvalenlab/deepcell-toolbox) for pre and post-processing the outputs of deep learning models, [DeepCell Tracking](https://github.com/vanvalenlab/deepcell-tracking) for creating cell lineages with deep-learning-based tracking models, and the [DeepCell Kiosk](https://github.com/vanvalenlab/kiosk-console) for deploying workflows on large datasets in the cloud. Additionally, we have developed [DeepCell Label](https://github.com/vanvalenlab/deepcell-label) for annotating high-dimensional biological images to use as training data.
        
        Read the documentation at [deepcell.readthedocs.io](https://deepcell.readthedocs.io).
        
        For more information on deploying models in the cloud refer to the [the Kiosk documentation](https://deepcell-kiosk.readthedocs.io).
        
        For TensorFlow 1.X or Python 2.7 support, please use deepcell [0.7.0](https://github.com/vanvalenlab/deepcell-tf/tree/0.7.0) or earlier.
        
        ## Examples
        
        <table width="700" border="1" cellpadding="5">
        
        <tr>
        <td align="center" valign="center">
        Raw Image
        </td>
        
        <td align="center" valign="center">
        Tracked Image
        </td>
        </tr>
        
        <tr>
        <td align="center" valign="center">
        <img src="https://raw.githubusercontent.com/vanvalenlab/deepcell-tf/master/docs/images/raw.gif" alt="Raw Image" />
        </td>
        
        <td align="center" valign="center">
        <img src="https://raw.githubusercontent.com/vanvalenlab/deepcell-tf/master/docs/images/tracked.gif" alt="Tracked Image" />
        </td>
        </tr>
        
        </table>
        
        ## Getting Started
        
        ### Install with pip
        
        The fastest way to get started with `deepcell-tf` is to install the package with `pip`:
        
        ```bash
        $ pip install deepcell
        ```
        
        ### Install with Docker
        
        There are also [docker containers](https://hub.docker.com/r/vanvalenlab/deepcell-tf) with GPU support available from DockerHub.
        run one of our existing images from Docker Hub. To  run the library locally on a GPU, you will need to make sure the latest version of <tt><a href="https://github.com/NVIDIA/nvidia-docker">nvidia-docker</a></tt> and [CUDA](https://developer.nvidia.com/cuda-downloads) are installed. Alternatively, Google Cloud Platform (GCP) offers prebuilt virtual machines preinstalled with Cuda, Docker, and the NVIDIA Container Toolkit.
        
        Once `nvidia-docker` is installed, run the following command:
        
        ```bash
        # Start a GPU enabled container on one GPUs
        docker run --gpus '"device=0"' -it --rm \
            -p 8888:8888 \
            -v $PWD/notebooks:/notebooks \
            -v $PWD/data:/data \
            vanvalenlab/deepcell-tf:0.9.0-gpu
        ```
        
        This will spin up a docker container with `deepcell-tf` installed and start a jupyter session using the default port 8888. This command also mounts a data folder (`$PWD/data`) and a scripts folder (`$PWD/scripts`) to the docker container so it can access data and Juyter notebooks stored on the host workstation. For any saved data or models to persist once the container is shut down, or be accessible outside of the container in general, it must be saved in these mounted directories. The default port can be changed to any non-reserved port by updating `-p 8888:8888` to, e.g., `-p 8080:8888`. If you run across any errors getting started, you should either refer to the `deepcell-tf` for developers section or raise an issue on GitHub.
        
        For examples of how to train models with the `deepcell-tf` library, check out the following notebooks:
        
        - [Training a segmentation model](https://deepcell.readthedocs.io/en/master/notebooks/Training-Segmentation.html)
        - [Training a tracking model](https://deepcell.readthedocs.io/en/master/notebooks/Training-Tracking.html)
        
        ## DeepCell Applications and DeepCell Datasets
        
        `deepcell-tf` contains two modules that greatly simplify the development and usage of deep learning models for single cell analysis. The first is <tt><a href="https://deepcell.readthedocs.io/en/master/API/deepcell.datasets.html">deepcell.datasets</a></tt>, which a collection of biological images that have single-cell annotations. These data include live-cell imaging movies of fluorescent nuclei (approximately 10,000 single-cell trajectories over 30 frames), as well as static images of whole cells (both phase and fluorescence images - approximately 75,000 single cell annotations). The second is <tt><a href="https://deepcell.readthedocs.io/en/master/API/deepcell.applications.html">deepcell.applications</a></tt>, which contains pre-trained models (fluorescent nuclear and phase/fluorescent whole cell) for single-cell analysis. Provided data is scaled so that the physical size of each pixel matches that in the training dataset, these models can be used out of the box on live-cell imaging data. We are currently working to expand these modules to include data and models for tissue images. Please note that they may be spun off into their own GitHub repositories in the near future.
        
        ## DeepCell-tf for Developers
        
        `deepcell-tf` uses `nvidia-docker` and `tensorflow` to enable GPU processing. If using GCP, there are [pre-built images](https://console.cloud.google.com/marketplace/details/nvidia-ngc-public/nvidia_gpu_cloud_image) which come with CUDA, docker, and nvidia-docker pre-installed. Otherwise, you will need to install [docker](https://docs.docker.com/install/linux/docker-ce/debian/), [nvidia-docker](https://github.com/NVIDIA/nvidia-docker), and [CUDA](https://developer.nvidia.com/cuda-downloads) separately. These instructions set up a docker container so that you can directly change the `deepcell-tf` library itself and have those changes reflected within the container
        
        ### Build a local docker container, specifying the tensorflow version with TF_VERSION
        
        ```bash
        git clone https://github.com/vanvalenlab/deepcell-tf.git
        cd deepcell-tf
        docker build --build-arg TF_VERSION=2.3.1-gpu -t $USER/deepcell-tf .
        ```
        
        ### Run the new docker image
        
        ```bash
        # '"device=0"' refers to the specific GPU(s) to run DeepCell-tf on, and is not required
        docker run --gpus '"device=0"' -it \
        -p 8888:8888 \
        $USER/deepcell-tf:latest-gpu
        ```
        
        It can also be helpful to mount the local copy of the repository and the notebooks to speed up local development. However, if you are going to mount a local version of the repository, you must first run the docker image without the local repository mounted so that the C extensions can be compiled and then copied over to your local version.
        
        ```bash
        # First run the docker image without mounting externally
        docker run --gpus '"device=0"' -it \
        -p 8888:8888 \
        $USER/deepcell-tf:latest-gpu
        
        # Use ctrl-p, ctrl-q (or ctrl+p+q) to exit the running docker image without shutting it down
        
        # Then, get the container_id corresponding to the running image of DeepCell-tf
        container_id=$(docker ps -q --filter ancestor="$USER/deepcell-tf")
        
        # Copy the compiled c extensions into your local version of the codebase:
        docker cp "$container_id:/usr/local/lib/python3.6/dist-packages/deepcell/utils/compute_overlap.cpython-36m-x86_64-linux-gnu.so" deepcell/utils/compute_overlap.cpython-36m-x86_64-linux-gnu.so
        
        # close the running docker
        docker kill $container_id
        
        # you can now start the docker image with the code mounted for easy editing
        docker run --gpus '"device=0"' -it \
            -p 8888:8888 \
            -v $PWD/deepcell:/usr/local/lib/python3.6/dist-packages/deepcell/ \
            -v $PWD/notebooks:/notebooks \
            -v /$PWD:/data \
            $USER/deepcell-tf:latest-gpu
        ```
        
        ## How to Cite
        
        - [Deep Learning Automates the Quantitative Analysis of Individual Cells in Live-Cell Imaging Experiments](https://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1005177)
        - [Dynamic allocation of computational resources for deep learning-enabled cellular image analysis with Kubernetes](https://www.biorxiv.org/content/10.1101/505032v3)
        - [Accurate cell tracking and lineage construction in live-cell imaging experiments with deep learning](https://www.biorxiv.org/content/10.1101/803205v2)
        
        ## Copyright
        
        Copyright © 2016-2021 [The Van Valen Lab](http://www.vanvalen.caltech.edu/) at the California Institute of Technology (Caltech), with support from the Shurl and Kay Curci Foundation, Google Research Cloud, the Paul Allen Family Foundation, & National Institutes of Health (NIH) under Grant U24CA224309-01.
        All rights reserved.
        
        ## License
        
        This software is licensed under a modified [APACHE2](https://github.com/vanvalenlab/deepcell-tf/blob/master/LICENSE). See [LICENSE](https://github.com/vanvalenlab/deepcell-tf/blob/master/LICENSE) for full details.
        
        ## Trademarks
        
        All other trademarks referenced herein are the property of their respective owners.
        
        ## Credits
        
        [![Van Valen Lab, Caltech](https://upload.wikimedia.org/wikipedia/commons/7/75/Caltech_Logo.svg)](http://www.vanvalen.caltech.edu/)
        
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Classifier: Topic :: Scientific/Engineering :: Image Recognition
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Classifier: Topic :: Software Development :: Libraries :: Python Modules
Requires-Python: >=3.6, <3.9
Description-Content-Type: text/markdown
Provides-Extra: tests
