Metadata-Version: 2.1
Name: hicstuff
Version: 1.0.6
Summary: General purpose stuff to generate and handle Hi-C data in its simplest form.
Home-page: https://github.com/koszullab/hicstuff
Author: lyam.baudry@pasteur.fr
License: GPLv3
Description: # hicstuff
        
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        A lightweight library that generates and handles Hi-C contact maps in either 2Dbedgraph or [instaGRAAL](https://github.com/koszullab/instaGRAAL) format. It is essentially a merge of the [yahcp](https://github.com/baudrly/yahcp) pipeline, the [hicstuff](https://github.com/baudrly/hicstuff) library and extra features illustrated in the [3C tutorial](https://github.com/axelcournac/3C_tutorial) and the [DADE pipeline](https://github.com/scovit/dade), all packaged together for extra convenience.
        
        ## Table of contents
        
        * [Installation](#Installation)
        * [Usage](#Usage)
          * [Full pipeline](#Full-pipeline)
          * [Individual components](#Individual-components)
        * [Library](#Library)
        * [Connecting the modules](#Connecting-the-modules)
        * [File formats](#File-formats)
        
        ## Installation
        
        To install a stable version:
        ```sh
           pip3 install -U hicstuff
        ```
        
        or, for the latest development version:
        
        ```sh
            pip3 install -e git+https://github.com/koszullab/hicstuff.git@master#egg=hicstuff
        ```
        
        Note for OSX and BSD users: `hicstuff pipeline` relies on the GNU coreutils. If you want to use it, you should use these as your default. [Here](https://www.topbug.net/blog/2013/04/14/install-and-use-gnu-command-line-tools-in-mac-os-x/) is a tutorial to set the gnu coreutils as default commands on OSX. 
        
        ## Usage
        
        ### Full pipeline
        
        All components of the pipelines can be run at once using the `hicstuff pipeline` command. This allows to generate a contact matrix from reads in a single command. By default, the output sparse matrix is in GRAAL format, but it can be a 2D bedgraph file if required.
        
            usage:
                pipeline [--quality_min=INT] [--duplicates] [--size=INT] [--no-cleanup]
                         [--threads=INT] [--minimap2] [--bedgraph] [--prefix=PREFIX]
                         [--tmpdir=DIR] [--iterative] [--outdir=DIR] [--filter]
                         [--enzyme=ENZ] [--plot] --fasta=FILE
                         (<fq1> <fq2> | --sam <sam1> <sam2> | --pairs <bed2D>)
        
            arguments:
                fq1:             Forward fastq file. Required by default.
                fq2:             Reverse fastq file. Required by default.
                sam1:            Forward SAM file. Required if using --sam to skip
                                 mapping.
                sam2:            Reverse SAM file. Required if using --sam to skip
                                 mapping.
                bed2D:           Sorted 2D BED file of pairs. Required if using
                                 "--pairs" to only build matrix.
        
        
            options:
                -b, --bedgraph                If enabled, generates a sparse matrix in
                                              2D Bedgraph format (cooler-compatible)
                                              instead of GRAAL-compatible format.
                -C, --circular                Enable if the genome is circular.
                -d, --duplicates:             If enabled, trims (10bp) adapters and
                                              remove PCR duplicates prior to mapping.
                                              Only works if reads start with a 10bp
                                              sequence. Not enabled by default.
                -e ENZ, --enzyme=ENZ          Restriction enzyme if a string, or chunk
                                              size (i.e. resolution) if a number. Can
                                              also be multiple comma-separated enzymes.
                                              [default: 5000]
                -f FILE, --fasta=FILE         Reference genome to map against in FASTA
                                              format
                -F, --filter                  Filter out spurious 3C events (loops and
                                              uncuts) using hicstuff filter. Requires
                                              "-e" to be a restriction enzyme, not a
                                              chunk size.
                -S, --sam                     Skip the mapping and start pipeline from
                                              fragment attribution using SAM files.
                -i, --iterative               Map reads iteratively using hicstuff
                                              iteralign, by truncating reads to 20bp
                                              and then repeatedly extending and
                                              aligning them.
                -m, --minimap2                Use the minimap2 aligner instead of
                                              bowtie2. Not enabled by default.
                -A, --pairs                   Start from the matrix building step using
                                              a sorted list of pairs in 2D BED format.
                -n, --no-cleanup              If enabled, intermediary BED files will
                                              be kept after generating the contact map.
                                              Disabled by defaut.
                -o DIR, --outdir=DIR          Output directory. Defaults to the current
                                              directory.
                -p, --plot                    Generates plots in the output directory
                                              at different steps of the pipeline.
                -P PREFIX, --prefix=PREFIX    Overrides default GRAAL-compatible
                                              filenames and use a prefix with
                                              extensions instead.
                -q INT, --quality_min=INT     Minimum mapping quality for selecting
                                              contacts. [default: 30].
                -s INT, --size=INT            Minimum size threshold to consider
                                              contigs. Keep all contigs by default.
                                              [default: 0]
                -t INT, --threads=INT         Number of threads to allocate.
                                              [default: 1].
                -T DIR, --tmpdir=DIR          Directory for storing intermediary BED
                                              files and temporary sort files. Defaults
                                              to the output directory.
        
            output:
                abs_fragments_contacts_weighted.txt: the sparse contact map
                fragments_list.txt: information about restriction fragments (or chunks)
                info_contigs.txt: information about contigs or chromosomes
        
        For example, to run the pipeline with minimap2 using 8 threads and generate a matrix in instagraal format in the directory `out`:
        ```
        hicstuff pipeline -t 8 -m -e DpnII -o out/ -f genome.fa reads_for.fq reads_rev.fq
        ```
        
        ### Individual components
        
        For more advanced usage, different scripts can be used independently on the command line to perform individual parts of the pipeline. This readme contains quick descriptions and example usages. To obtain detailed instructions on any subcommand, one can use `hicstuff <subcommand> --help`.
        
        #### Iterative alignment
        
        Truncate reads from a fastq file to 20 basepairs and iteratively extend and re-align the unmapped reads to optimize the proportion of uniquely aligned reads in a 3C library.
        
            usage:
                hicstuff iteralign [--minimap2] [--threads=1] [--min_len=20] --out_sam=FILE --fasta=FILE <reads.fq>
        
        #### Digestion of the genome
        
        Digests a fasta file into fragments based on a restriction enzyme or a
        fixed chunk size. Generates two output files into the target directory
        named "info_contigs.txt" and "fragments_list.txt"
        
            usage:
                digest [--plot] [--figdir=FILE] [--circular] [--size=INT]
                       [--outdir=DIR] --enzyme=ENZ <fasta>
        
         
         For example, to digest the yeast genome with MaeII and HinfI and show histogram of fragment lengths:
        
        `hicstuff digest --plot --outdir output_dir --enzyme MaeII,HinfI Sc_ref.fa`
        
        #### Filtering of 3C events
        
        Filters spurious 3C events such as loops and uncuts from the library based
        on a minimum distance threshold automatically estimated from the library by default. Can also plot 3C library statistics.
        
            usage:
                filter [--interactive | --thresholds INT-INT] [--plot]
                       [--figdir FILE] <input> <output>
        
        #### Viewing the contact map
        
        Visualize a Hi-C matrix file as a heatmap of contact frequencies. Allows to tune visualisation by binning and normalizing the matrix, and to save the output image to disk. If no output is specified, the output is displayed interactively. If two contact maps are provided, the log ratio of the first divided by the second will be shown.
        
            usage:
                view [--binning=1] [--despeckle] [--frags FILE] [--trim INT]
                     [--normalize] [--max=99] [--output=IMG] [--cmap=CMAP]
                     [--log] [--region=STR] <contact_map> [<contact_map2>]
        
        For example, to view a 1Mb region of chromosome 1 from a full genome Hi-C matrix rebinned at 10kb:
        
        ```sh
            hicstuff view --normalize --binning 10kb --region chr1:10,000,000-11,000,000 --frags fragments_list.txt contact_map.tsv
        ```
        ### Library
        
        All components of the hicstuff program can be used as python modules. See the documentation on [reathedocs](https://hicstuff.readthedocs.io). The expected contact map format for the library is a simple CSV file, and the objects handled by the library are simple ```numpy``` arrays. The various submodules of hicstuff contain various utilities.
        
        ```python
        import hicstuff.digest # Functions to work with fragments (digestion, matrix building)
        import hicstuff.iteralign # Functions related to iterative alignment
        import hicstuff.hicstuff # Contains utilities to modify and operate on contact maps as numpy arrays
        import hicstuff.filter # Functions for filtering 3C events by type (uncut, loop)
        import hicstuff.view # Utilities to visualise contact maps
        ```
        
        ### Connecting the modules
        
        All the steps described here are handled automatically when running the `hicstuff pipeline`. But if you want to connect the different modules manually, the intermediate input and output files must be processed using light bash scripting.
        
        #### Extracting contacts from the alignment
        
        The output from iteralign is a SAM file. In order to retrieve Hi-C pairs, you need to run iteralign separately on the two fastq files and process the resulting alignment files processed as follows using bedtools and some bash commands.
        
        1. Convert the SAM files into BED format
        
        ```bash
        samtools view -bS -F 260 -@ $t -q 30 "for.sam" |
          bedtools bamtobed -i - |
          awk 'OFS="\t" { print $1,$2,$3,$4,$6 }' \
            > contacts_for.bed
        
        samtools view -bS -F 260 -@ $t -q 30 "rev.sam" |
          bedtools bamtobed -i - |
          awk 'OFS="\t" { print $1,$2,$3,$4,$6 }' \
            > contacts_rev.bed
        ```
        
        2. Put all forward and reverse reads into a single sorted BED file
        
        ```bash
        sort -k1,1d -k2,2n contacts_for.bed contacts_rev.bed \
          > total_contacts.bed
        
        ```
        
        #### Attributing each read to a restriction fragment
        To build a a contact matrix, we need to attribute each read to a fragment in the genome. This is done by intersecting all the reads with the digested genome.
        
        1. Extract restriction fragments from the genome using `hicstuff digest`.
        
        ```bash
        # Generate fragments_list.txt
        hicstuff digest --fasta genome.fa \
          --enzyme DpnII \
          --output-dir .
        
        # Make a BED from it
        awk 'NR>1 { print $2"\t"$3"\t"$4"\t"(NR-2) }' fragments_list.txt
          >fragments_list.bed
        
        ```
        
        2. Intersect the BED files of reads and restriction fragments.
        
        ```bash
        bedtools intersect -a total_contacts.bed \
          -b fragments_list.bed -wa -wb |
          sort -k4d  \
          > contact_intersect_sorted.bed
        ```
        
        4. Get reads into a paired BED file (1 pair per line)
        
        ```bash
        # Note: F is fragment and R is read
        # This awk snippet allows to convert a sorted BED file with fields:
        #   Rchr Rstart Rend Rname Rstrand Fchr Fstart Fend Fidx
        # to a "2D BED" file with:
        #   F1chr F1start F1end F1idx R1strand F2chr F2start F2end F2idx R2strand
        bed2pairs='
        BEGIN{dir="for"; OFS="\t"}
        {
          if(dir=="for") {
            fw["name"]=$4; fw["coord"]=$6"\t"$7"\t"$8"\t"$9"\t"$5; dir="rev" }
          else {
            if(fw["name"] == $4) {
                print fw["coord"],$6,$7,$8,$9,$5; dir="for"}
            else {
                dir="rev"; fw["coord"]=$6"\t"$7"\t"$8"\t"$9"\t"$5; fw["name"]=$4}
            }
        }
        '
        awk "$bed2pairs" contact_intersect_sorted.bed > contact_intersect_sorted.bed2D
        ```
        
        The resulting 2D BED file can then be filtered by the `hicstuff filter` module if needed, otherwise, the matrix can be built directly from it. To generate a GRAAL-compatible sparse matrix from the 2D bed file:
        
        ```bash
        # Remove strand information, sort by fragment combination,
        # Count occurrences of each fragment combination and format into csv.
        echo -e "id_fragment_a\tid_fragment_b\tn_contact" > matrix.tsv
        cut -f4,9 "$tmp_dir/contact_intersect_sorted.bed2D" |
          sort -V |
          uniq -c |
          sed 's/^ *//' |
          tr ' ' '\t' |
          awk '{print $0,$1}' |
          cut -f1 --complement >> matrix.tsv
        ```
        
        ### File formats
        
        * 2D BED: This is the input format for `hicstuff filter`. It has one line per Hi-C pair and the following fields for each read: **chr start end name frag_id strand**.
        * 2D bedgraph: This is an optional output format of `hicstuff pipeline` for the sparse matrix. It has two fragment per line, and the number of times they are found together. It has the following fields: **chr1, start1, end1, chr2, start2, end2, occurences**
        
        * GRAAL sparse matrix: This is a simple CSV file with 3 columns: **id_a, id_b, occurrences**. The id columns correspond to the absolute id of the restriction fragments (0-indexed).
        
Platform: UNKNOWN
Classifier: Development Status :: 3 - Alpha
Classifier: Intended Audience :: Science/Research
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Classifier: License :: OSI Approved :: GNU General Public License v3 (GPLv3)
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Classifier: Programming Language :: Python :: 3.7
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