1.CHROM : Chromosome
2.POS : Position
3.REF ： Reference allele
4.ALT : Alternative allele
5.wild.AF : wild bulk alternative allele frequency (the same as snp-index)
6.mutant.AF : mutant bulk alternative allele frequency (the same as snp-index)
7.AFD(deltaSNP) : Alternative allele frequency difference (mutant.AF - wild.AF)
8.REF_FRQ : total ref allele coverage of two bulk / total coverage (both refer and allele) of two bulk
9.wild.DP : wild bulk read coverage depth
10.mutant.DP : mutant bulk read coverage depth
11.ED : Euclidean distance at each SNP
12.fitted : ED^4 fit using a Loess curve
13.unfitted : ED^4
14.dis2edcutoff : cutoffs conservatively with a minimum distance of 0.5 and a minimum mutant pool allele frequency of 0.75.
15.Feature ： Variant impact type
16.Gene : Gene ID on this locus
17.Transcript : Transcript ID on this locus


Citation:
Gorsi, B., Yost, H. J., Demarest, B. L., Bisgrove, B. W., Hill, J. T., & Su, Y.-C. (2013). MMAPPR: Mutation Mapping Analysis Pipeline for Pooled RNA-seq. Genome Research, 23(4), 687–697. https://doi.org/10.1101/gr.146936.112